Antioxidant Activity of Flavonoids and Phenolic Acids from Dodonaea angustifolia Flower: HPLC Profile and PASS Prediction

Background. Dodonaea angustifolia is a known medicinal plant across East Arica. The flower of D. not well investigated in terms phytochemistry and biological activities. This study aims to investigate the presence flavonoids phenolic acids its antioxidant activity. Methods. Preliminary phytochemical screening was carried out using standard protocols. Antioxidant activity evaluation DPPH assay total phenol content (TPC) flavonoid (TFC) determinations extract were compared with values leaf extract. UHPLC-DAD analysis managed develop profile Prediction spectra for substances (PASS) done an online server related Results. TPC TFC confirmed acids. From HPLC flavonoids, quercetin, myricetin, rutin, such as chlorogenic acid, gallic syringic acid detected quantified. spectrum predicted quantified polyphenols. Conclusions. rich source acids, which are extractable can be checked further It possible identify quantify compounds through methanol flower. PASS prediction results showed that there stronger activities identified flavonoids. Future work will emphasize isolation characterization active principles responsible bioactivity.